Method Setup

Selected HPLC method metadata

Method template

Column chemistry

Column brand / method template

Mobile phase A

Mobile phase B

Mobile phase C

Additive

Mode

Detector

Column length (mm)

Column I.D. (mm)

Particle size (um)

Flow rate (mL/min)

Temperature (deg C)

Sample dissolution solvent

Run time (min)

Method optimization

Scouting-run assisted gradient, flow, and temperature selection

Maximize Rs Minimize run time Maximize Pc

Fast scouting tR (min)

Fast gradient time (min)

Fast %B window

Slow scouting tR (min)

Slow gradient time (min)

Slow %B window

Select an objective, optionally enter two scouting retention times, then run Optimize Method.

Advanced Instrument Geometry

Dwell volume (mL)

Injection volume (uL)

Column porosity

Tubing volume (uL)

Tubing I.D. (um)

Tubing length (cm)

Flow cell volume (uL)

Injection valve volume (uL)

Gradient controls

Shown only for gradient mode

Start %B

End %B

Gradient time (min)

Hold time (min)

Line C constant (%)

Enter gradient values to review curve slope, composition window, and method timing.

Instrument guardrails

Estimated from flow, column, particle size, temperature, and mobile phase

Complete method fields to estimate maximum backpressure.

Complete column and gradient fields to estimate column-volume turnover.

Method notes

Editable interpretation text carried into print/export

Prediction Run

Prepare method-specific prediction payload

Simulated Chromatogram

Predicted chromatogram

Simulated chromatogram based on selected method. The rendered peak shape is generated in-browser and is not raw instrument output.

Chromatographic metrics

Resolution, peak capacity, and EMG-derived shape factors

Critical Rs Awaiting run

Peak capacity Pc Awaiting run

Tailing / Asymmetry Awaiting run

Run a prediction to populate neighboring-peak resolution metrics.

Prediction Payload

Future-ready integration object

No payload generated yet.

ChemrytHPLC

Predicted retention and method interpretation